Chelsea and Manchester United will face each other in the quarter-finals of the Champions League after being drawn together – while Tottenham will play Real Madrid.
Barcelona meet Shakhtar Donetsk, with reigning champions Inter Milan drawn against Schalke.
Chelsea or United will go on to face either Inter or Schalke, while the winners of the other two last eight ties will meet in the other semi-final.
The quarter-final ties will be played on April 5, 6, 12 and 13.
The Blues have not yet won the Champions League and will be out for revenge after losing the 2008 Champions League final to United on penalties in Moscow – when John Terry famously missed his spot-kick.
The first leg between the Premier League rivals will take place at Stamford Bridge with United, who have won Europe’s top competition three times, at home for the second leg.
Chelsea secretary David Barnard said: “We have a great rivalry domestically but the Champions League is something different.
“We’re looking forward to it, people will bring up Moscow and revenge but we know each other well enough.
“Most of that team that experienced Moscow are still here so it’ll be a great motivation. We’ve been the bridesmaid many times.”
The tie between Chelsea and United means at least one English team is guaranteed a place in the semi-finals of the Champions League and the chance to make it to the final at Wembley on 28 May.
Whoever does win will play the first leg of their semi-final against Inter or Schalke away with the return at home.
United secretary John Alexander said: “It’s a shame the English clubs were paired together. We have a good record against domestic clubs in Europe and I’m sure [manager] Sir Alex [Ferguson] will be prepared.
“It’s a great chance for whoever comes through to make it to Wembley. Most teams wanted to avoid a clash with Barcelona.”
Spurs beat AC Milan in the last round and will travel to the Bernabeu for the first leg of their tie against Real Madrid.
Darren Eales, Spurs director of football administration, said: “It really is a dream tie for us, they’ve won this competition more than anyone else.
“It’s a game of football so anything can happen.”